Nucleic Acid Extraction and Purification: Chaotropic agent that lyses cells and promotes DNA/RNA binding to silica or magnetic beads while removing proteins and polysaccharides.

DNA/RNA Denaturation and Hybridization: Controls stringency for Southern/Northern blots and in situ hybridization and supports DNA melting curve analyses.

PCR Product and Plasmid Cleanup: Provides high-salt conditions for binding nucleic acids to spin columns prior to wash and elution steps.

Phenol/Chloroform Extractions: Enhances phase separation and recovery of high-quality nucleic acids during organic extractions.

Anion-Exchange Chromatography (Nucleic Acids): Used for high-salt elution of DNA/RNA from DEAE or Q resins.

Protein-Nucleic Acid Complex Disruption: Denatures proteins and dissociates ribonucleoprotein and chromatin complexes to improve nucleic acid yield and purity.