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2R0625

Assay Diluent. 2L, Sterile

Availability

Ships in 6 - 14 Days

Container Size

2L

Item Count

1 Bottle(s)

Price per Item

$209.00

Fill Volume

2000 mL

COA
COA

2R0625

Assay Diluent. 2L, Sterile

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

Assay Diluent is a ready-to-use phosphate-buffered saline with 0.5% BSA and 0.05% polysorbate 20 (Tween-20). The 2 L fill supports high-throughput plate work.

It is commonly used to dilute samples, standards, and detection antibodies in ELISAs and other immunoassays, including host cell protein (HCP) and residual Protein A assays for biologics. BSA and surfactant reduce nonspecific binding and matrix effects.

  • 135.0mM Sodium Chloride
  • 2.7mM Potassium Chloride
  • 4.3mM Disodium Phosphate
  • 1.4mM Monopotassium Phosphate
  • 0.50% Bovine Serum Albumin
  • 0.05% Tween-20
  • ELISA Diluent: Diluent for samples, standards, and detection antibodies in HCP, residual Protein A, and binding ELISAs.
  • ELISA Plate Blocking: Ready-to-use blocking buffer for occupying unbound sites on coated plates to lower background.
  • ELISA Plate Washing: Protein-containing buffer for low-background wash steps in ELISAs.
  • Bead-Based and Multiplex Immunoassays: Buffer for dilution, blocking, and washing in bead-based and multiplex immunoassays.
  • Lateral Flow Assays: Running and sample buffer for lateral flow and rapid immunoassays.
  • Protein and Enzyme Stabilization: BSA-containing buffer that stabilizes dilute proteins and antibodies during handling and storage.
  • Flow Cytometry: Protein-containing buffer for antibody staining and washing of cells for flow cytometry.
  • Immunoassay Standards and Calibrators: Diluent for preparing immunoassay standards, calibrators, and controls.
  • Antibody Dilution and Storage: Protein-containing diluent for primary and secondary antibodies and conjugates in immunodetection.
  • Immunohistochemistry and Immunofluorescence: Protein-containing buffer for blocking and antibody dilution in IHC, ICC, and IF.
Storage Temperature
Refrigerated
Shelf Life
180 days
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