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2A3030

HEPES Assay Diluent, pH 7.5. 1000mL, Sterile

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$162.00

Fill Volume

1000 mL

2A3030

HEPES Assay Diluent, pH 7.5. 1000mL, Sterile

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

HEPES Assay Diluent, pH 7.5 is a HEPES-buffered saline formulated for the preparation and dilution of samples, standards, and conjugates in immunoassay workflows. The buffer maintains physiological ionic strength and includes a nonionic surfactant and a protein additive for consistent handling of biological reagents.

HEPES provides stable pH buffering near neutrality without reliance on CO₂ equilibration, supporting reproducible conditions during assay setup and washing steps.

  • 0.59% HEPES Free Acid
  • 0.65% HEPES Sodium Salt
  • 0.90% Sodium Chloride
  • 0.05% Tween-20
  • 1.00% Bovine Serum Albumin (BSA)
  • ELISA Assay Diluent: Dilutes samples, standards, and controls while minimizing nonspecific binding via BSA and Tween-20 at pH 7.5.

  • Antibody Diluent (Primary/Secondary): Stabilizes antibodies and reduces background during incubation steps.

  • Plate Blocking/Stabilization: Provides protein-based blocking and antigen/antibody stabilization on microplates.

  • Wash/Carry Buffer for Immunoassays: Maintains ionic strength and reduces hydrophobic interactions for consistent washes in ELISA and bead assays.

  • Lateral Flow/Immunochromatography Running Buffer: Supports consistent flow and minimizes nonspecific interactions in strip-based tests.

  • Flow Cytometry Staining Buffer: Dilutes labeled antibodies and cells to lower background and improve signal specificity.

  • Bead-Based Multiplex Assays: Used as a sample and reagent diluent for Luminex-style immunoassays to improve precision and recovery.

  • ELISpot/Cell-Based Immunoassays: Serves as a gentle, protein-containing diluent for capture/detection reagents and samples.

  • Protein/Antigen Sample Dilution: Dilutes recombinant proteins or lysates (e.g., Escherichia coli, Staphylococcus aureus) while preserving activity and reducing nonspecific adsorption.

  • Standard Curve Preparation: Provides a consistent matrix for serial dilutions to ensure linear, reproducible calibration.

  • pH: 7.50 ± 0.04
Storage Temperature
Refrigerated
Shelf Life
180 days
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