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23915

Tryptic Soy Agar Settle Plates with Lecithin and Tween-80. 100mm, 5 Plates per Sleeve, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

100mm

Item Count

5 Plates

Price per Item

$15.00

Fill Volume

25 mL

23915

Tryptic Soy Agar Settle Plates with Lecithin and Tween-80. 100mm, 5 Plates per Sleeve, Sterile.

Quantity

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Description
Formulation
Common Applications
Storage

Tryptic Soy Agar Settle Plates with Lecithin and Tween-80 are general-purpose nutrient agar plates for passive air monitoring and surface exposure in cleanrooms and other controlled environments. The medium is based on casein and soybean peptones with sodium chloride and agar, and is supplemented with lecithin and polysorbate 80 (Tween-80) to aid in the neutralization of residual disinfectants and cleaning agents during environmental sampling.

These plates are used for the recovery of viable aerobic microorganisms from air or exposed surfaces in environmental monitoring programs and method suitability studies. Commonly referenced challenge organisms include Staphylococcus aureus, Escherichia coli, and Bacillus subtilis, along with environmental bacterial and fungal isolates.

  • 1.5% Tryptone
  • 0.5% Soytone
  • 0.5% Sodium Chloride
  • 1.5% Agar
  • 0.07% Lecithin
  • 0.50% Tween-80
  • Environmental Monitoring (Passive Air/Settle Sampling): Placed open in cleanrooms and production areas to capture viable airborne fallout on TSA (1.5% Tryptone, 0.5% Soytone, 0.5% NaCl, 1.5% Agar) for enumeration and trending.

  • Aseptic Process Simulation (Media Fills): Deployed around critical zones during media fills to assess microbial fallout during simulated operations.

  • HVAC and Facility Qualification: Used during commissioning and requalification to monitor microbial deposition associated with airflow patterns and pressure differentials.

  • Routine Cleanroom EM Trending: Positioned at defined locations to track baseline and excursion events across ISO-classified areas.

  • Disinfectant Neutralization and Recovery Studies: Lecithin (0.07%) and Tween-80 (0.50%) neutralize quaternary ammonium compounds and surfactants, enabling recovery of disinfectant-exposed microbes.

  • Post-Cleaning Effectiveness Verification: Placed during and after sanitization to verify reduction of viable fallout while allowing recovery in the presence of residual sanitizers.

  • Personnel Shedding Assessment (Proximity Monitoring): Set near operators and gowning areas to gauge microbial shedding into the environment during routine tasks.

  • Airborne Bacteria Surveillance: Supports broad recovery of aerobes such as Staphylococcus, Micrococcus, and Bacillus from ambient air.

  • Pharmaceutical and Biotech Production Monitoring: Used in sterile and non-sterile manufacturing to comply with EM programs and regulatory expectations.

  • Food and Beverage Facility Hygiene Monitoring: Deployed in processing/packaging areas to track microbial fallout that may impact product quality.

Storage Temperature
Room Temperature
Shelf Life
300 days
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