Isopycnic Density Gradient Centrifugation: Creates CsCl gradients for precise separation of nucleic acids by buoyant density.

Plasmid DNA Purification: Bands covalently closed circular plasmids away from genomic DNA and RNA from Escherichia coli lysates.

Viral and Phage Genome Purification: Isolates viral particles and genomes, including bacteriophages, for downstream analysis.

DNA/RNA Fractionation for Sequencing and Cloning: Separates ccc, nicked, linear DNA, and RNA for high-purity prep prior to sequencing or cloning.

Stable Isotope Probing (SIP): Resolves 13C/15N-labeled DNA from unlabeled DNA in microbial ecology studies (e.g., communities including Bacillus subtilis).

Organelle DNA Isolation: Enriches mitochondrial and chloroplast DNA from eukaryotic samples.

Analytical Ultracentrifugation: Determines nucleic acid buoyant densities and estimates GC content using CsCl equilibrium gradients.