Plasmid DNA Purification (CsCl Density Gradient): Cesium Chloride (1.3 g/mL) enables isopycnic ultracentrifugation of plasmid DNA from Escherichia coli, yielding highly pure, supercoiled DNA.

Viral Vector Purification: Used to form stable density gradients for purification of AAV, adenovirus, and bacteriophages from host lysates.

Genomic DNA/RNA Separation: Supports equilibrium gradients to separate and purify high–molecular weight genomic DNA and RNA based on buoyant density.

Endotoxin Reduction in DNA Preps: Classic CsCl–EtBr methods with Cesium Chloride (1.3 g/mL) reduce protein, RNA, and endotoxin contaminants in plasmid preparations.

Buoyant Density and GC-Content Analyses: Resolves DNA species by buoyant density for applications such as isotope labeling studies and GC-content profiling.

Bacteriophage Isolation: Concentrates and purifies phage particles propagated in Escherichia coli using isopycnic gradients.

Exosome and Vesicle Enrichment (Research Use): Employed in some workflows to separate extracellular vesicles by density during ultracentrifugation.