Density Gradient Ultracentrifugation: Cesium Chloride (1.5 g/mL) enables isopycnic separation of nucleic acids by buoyant density.

Plasmid DNA Purification: CsCl–ethidium bromide gradients isolate supercoiled plasmids from Escherichia coli lysates.

Viral Particle Purification: Purifies AAV, adenovirus, and bacteriophage using CsCl step or continuous gradients.

Phage DNA Preparation: Obtains high-purity bacteriophage DNA from infected Escherichia coli cultures.

Separation of DNA Topological Forms: Resolves supercoiled, nicked, and linear DNA species by buoyant density.

Mitochondrial and Genomic DNA Isolation: Enriches mtDNA and high–molecular-weight gDNA away from proteins and RNA.

RNA Cleanup: CsCl cushions remove proteins and polysaccharides from total RNA preparations.

Endotoxin Reduction in DNA Preps: Lowers LPS contamination during plasmid purification workflows.

Quality Control of Nucleic Acids: Assesses sample purity by buoyant density and refractive index in CsCl gradients.

Buoyant Density Standards: Serves as a density calibration medium for analytical ultracentrifugation.