Density Gradient Centrifugation: Employed as a high-density medium for isopycnic and rate-zonal separations of biomolecules and particles.

Viral Vector Purification: Used to purify AAV, adenovirus, and lentivirus preparations with minimal loss of infectivity.

Extracellular Vesicle Isolation: Facilitates enrichment of exosomes and microvesicles from cell culture supernatants and biofluids.

Lipoprotein Fractionation: Enables separation of HDL, LDL, and VLDL from serum or plasma.

Organelle and Membrane Isolation: Supports purification of nuclei, mitochondria, endosomes, peroxisomes, and detergent-free membrane rafts.

Protein Complex and VLP Purification: Provides a gentle medium for isolating sensitive protein assemblies and virus-like particles.

Cell Subpopulation Separation: Assists in density-based enrichment of PBMCs, leukocytes, and other primary cells.

Nuclei Preparation for Genomics: Used to obtain clean nuclei for ATAC-seq, CUT&RUN, and similar workflows.

Microbial Fractionation: Applied for density-based separation of bacterial cells such as Escherichia coli (E. coli) from lysates or debris.

Cushioning for Ultracentrifugation: Serves as a cushion layer to protect labile particles during high-speed spins.