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21431

25% Iodixanol with Phenol Red, 250mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$356.00

Fill Volume

250 mL

21431

25% Iodixanol with Phenol Red, 250mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Storage

25% Iodixanol with Phenol Red is a buffered, isotonic density medium formulated for laboratory preparation of aqueous density gradients. The inclusion of a pH indicator provides visible color for monitoring handling and dilution while maintaining physiological pH.

This solution is intended for creating continuous or discontinuous gradients used in the fractionation of viruses, extracellular vesicles, organelles, membranes, and macromolecular complexes by isopycnic or rate-zonal centrifugation. It can be used as a stock for dilution to target densities appropriate to specific sample types and protocols.

  • 1X PBS
  • 1mM Magnesium Chloride
  • 2.5mM Potassium Chloride
  • 25% Iodixanol
  • 0.001% Phenol Red
  • Density Gradient Ultracentrifugation: Isopycnic separation using iodixanol gradients for viruses, vesicles, and macromolecular complexes.

  • Viral Vector Purification: Purifies AAV, adenovirus, and lentivirus with high recovery and low toxicity for downstream applications.

  • Extracellular Vesicle (EV)/Exosome Isolation: Enriches EVs from cell culture media and biofluids with minimal protein contamination.

  • Organelle Fractionation: Isolates nuclei, mitochondria, endosomes, Golgi, and synaptosomes while preserving functionality.

  • Lipid Raft/Membrane Microdomain Isolation: Detergent-free buoyant density separation of cholesterol-rich membrane microdomains.

  • Bacterial Outer Membrane Vesicles (OMVs): Enriches OMVs from Escherichia coli and other gram-negative bacteria for proteomic and immunologic studies.

  • Nuclei Preparation for Genomics: Generates clean nuclei for ATAC-seq, CUT&RUN, and single-nucleus RNA-seq workflows.

  • Cell and Organoid Isolation by Density Cushion: Gentle separation of cells, organoids, and debris using iodixanol cushions.

  • Subcellular Proteomics/Metabolomics Prep: Produces clean subcellular fractions for unbiased omics analyses.

  • Vesicular Transport/Endosomal Trafficking Studies: Resolves early/late endosomes and lysosomes by buoyant density.

Storage Temperature
Room Temperature
Shelf Life
730 days
AAV Iodixanol Gradient Ultracentrifugation Workflow

This product is part of our AAV Iodixanol Gradient Ultracentrifugation Workflow

Get all the AAV reagents you need from one trusted source, and learn how our AAV·Tek™️ Solutions can save months in your process development.

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