Density Gradient Ultracentrifugation: Forms iso-osmotic gradients for purification of viral vectors (e.g., AAV, lentivirus), extracellular vesicles, and other nanoparticles.

Organelle Isolation: Separates nuclei, mitochondria, endosomes, and other subcellular fractions with minimal osmotic stress.

Cushion Centrifugation: Acts as an iodixanol cushion to concentrate and clean viral preparations and vesicles while reducing shear damage.

Extracellular Vesicle (EV) and Exosome Purification: Enables high-purity isolation from cell culture media and biofluids via step or continuous gradients.

Lipoprotein Fractionation: Resolves VLDL, LDL, and HDL based on buoyant density for lipidomics and cardiovascular research.

Membrane Microdomain/Raft Isolation: Preserves membrane integrity during detergent-free separation of lipid rafts and membrane proteins.

Protein Complex Stabilization: Maintains near-physiological osmolarity and density during centrifugation to protect labile protein assemblies.

Virus and Phage Purification: Provides reproducible banding for purification and titering of non-enveloped and enveloped viruses and bacteriophages.

Cell and Nuclei Cleanup: Removes debris and dead cells post-lysis or isolation while preserving morphology and function.

Nanoparticle and Liposome Separation: Resolves synthetic nanoparticles and liposomes by buoyant density for formulation and delivery studies.