Anion-Exchange Plasmid Elution: High-salt elution of plasmid DNA from anion-exchange columns/cartridges (50 mM Tris/Tris-HCl, 10 mM EDTA, 0.85 M NaCl).

Midiprep/Maxiprep Workflows: Recovery of pDNA from cleared alkaline lysates of E. coli during gravity-flow or FPLC-based purification.

Large Construct Recovery: Efficient elution of low-copy or large vectors (e.g., BACs/cosmids) requiring elevated ionic strength.

Endotoxin-Reduction Protocols: High-salt elution/wash steps prior to desalting or buffer exchange for low-endotoxin pDNA preps.

DNA Stabilization During Elution: EDTA chelation limits nuclease activity; Tris maintains pH 7.2 for pDNA integrity.

Downstream Applications (Post-Desalting): Provides eluted pDNA for cloning, restriction digest, PCR, and sequencing after salt/buffer exchange.