21231

pDNA Equilibration Buffer, pH 7.2, 500mL, Sterile

Availability

Ships in 6 - 14 Days

Container Size

650mL

Item Count

1 Bottle(s)

Price per Item

$57.00

Fill Volume

500 mL

See label

COA

21231

pDNA Equilibration Buffer, pH 7.2, 500mL, Sterile

Quantity

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Description

Formulation

Common Applications

Storage

pDNA Equilibration Buffer, pH 7.2, is a ready-to-use buffer intended for equilibrating chromatography media prior to plasmid DNA purification. It is used to establish a stable pH environment on anion-exchange or silica-based columns before loading cleared lysates.

Typical applications include preparing gravity-flow, spin, or FPLC columns for processing plasmid DNA from E. coli cultures. The buffer is applied after column preparation or regeneration and before wash and elution steps to maintain consistent conditions throughout the workflow.

50mM Tris
10 mM EDTA

Anion-Exchange Column Equilibration: Pre-equilibrates anion-exchange resins for plasmid DNA purification from E. coli lysates.
FPLC/Cartridge Conditioning: Conditions anion-exchange cartridges on systems like ÄKTA prior to sample loading.
Lysate Buffer Adjustment: Dilutes and adjusts cleared alkaline lysates to optimal pH/ionic strength before chromatography.
Pre-Elution Wash Steps: Used in wash sequences to remove RNA, proteins, and endotoxins prior to plasmid elution.
Plasmid Stability During Processing: 50 mM Tris, 10 mM EDTA maintain pH and chelate divalent cations to protect pDNA from nucleases.
Endotoxin-Aware Workflows: Supports low-endotoxin plasmid prep protocols for transfection-grade pDNA.

Storage Temperature

Room Temperature

Shelf Life

1095 days

Plasmid Workflow

This product is part of our Plasmid Workflow

To expedite your plasmid production, we offer a wide variety of high-quality off-the-shelf and custom solutions, from selective culture media to buffers and reagents that can be used throughout the pDNA growth and purification process.

Selection and Culturing
Purification and Recovery

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pDNA Equilibration Buffer, pH 7.2, 500mL, Sterile

Certificate of Analysis

This product is part of our Plasmid Workflow

Looking for a Certificate of Analysis?

A trusted partner that

can help you scale

Raising the bar on quality

Delivery in weeks, not months

Add our expertise to your team

We're custom by default

Making solutions possible