Plasmid DNA Purification: Wash step in alkaline lysis workflows to clean pDNA using a high-salt Tris/EDTA buffer (50 mM Tris, 10 mM EDTA, 0.6 M NaCl, pH 7.2).

Silica Spin Column Wash: Removes proteins, polysaccharides, and metabolites from silica membranes prior to elution.

Removal of Residual Chaotropes and Detergents: Clears guanidinium and SDS carryover to improve downstream performance.

Endotoxin Reduction: Minimizes endotoxin contamination in plasmid preps for sensitive cell-based applications.

Nuclease and RNA Clearance: Helps eliminate nucleases and RNA to yield cleaner pDNA.

Transfection-Ready DNA: Prepares high-purity plasmids for efficient transfection into mammalian cells.

Cloning and Restriction Digests: Provides clean plasmid DNA for ligations and restriction enzyme workflows.

PCR and qPCR: Ensures inhibitor-free pDNA suitable for amplification assays.

Sequencing (Sanger/NGS) Preparation: Reduces impurities that interfere with library prep and read quality.

Bead-Based Plasmid Cleanup: Washes magnetic bead-bound pDNA in manual or automated extraction protocols.

Microbial Plasmid Workflows: Used with plasmids propagated in E. coli prior to transformation or downstream analysis.