Plasmid DNA Purification: Used as a wash step during alkaline lysis preps of Escherichia coli cultures to remove proteins, salts, and other lysate contaminants prior to elution.

Endotoxin Reduction: Helps reduce lipopolysaccharide (LPS) carryover to produce transfection-grade plasmid DNA for sensitive cell-based applications.

Desalting and Cleanup: Removes residual chaotropes, detergents, and salts to improve downstream enzymatic performance.

Silica Column Workflows: Serves as a high-salt wash in spin columns and gravity columns for reliable plasmid cleanup.

Magnetic Bead Workflows: Functions as a wash buffer in bead-based plasmid DNA purification protocols.

Restriction/Ligation Prep: Prepares purified plasmid DNA for restriction digestion and ligation by minimizing inhibitory contaminants.

PCR and qPCR Prep: Ensures cleaner plasmid templates by reducing inhibitors that affect polymerase activity.

Cloning and Sequencing: Improves read quality and cloning efficiency by lowering carryover of proteins, RNA, and genomic DNA.