Plasmid DNA Miniprep Neutralization: 3M Potassium Acetate (pH 5.5) neutralizes alkaline lysates of E. coli, precipitating SDS, proteins, and genomic DNA while keeping plasmids in solution.

RNA Purification: Acidic acetate conditions help remove proteins and DNA contaminants and stabilize RNA during extraction.

Nucleic Acid Precipitation: Provides potassium ions and acetate for efficient DNA/RNA precipitation with ethanol or isopropanol.

Endotoxin and Debris Removal: Facilitates precipitation of lipopolysaccharides and cellular debris from bacterial lysates (e.g., Escherichia coli).

Protein Cleanup: Used to precipitate proteins and reduce polysaccharide carryover in clarified lysates.

Acetate Buffer Preparation: Serves as a sterile stock to make acetate buffers at pH 5.5 for biochemical workflows.

Enzyme Assays at Mildly Acidic pH: Maintains consistent pH 5.5 conditions for enzymes requiring acetate buffering.

Sample Clarification Prior to Chromatography: Aids in removing precipitable contaminants to protect downstream columns.