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21221

3M Potassium Acetate, pH 5.5, 100mL, Sterile

Availability

Ships in 6 - 14 Days

Container Size

125mL

Item Count

1 Bottle(s)

Price per Item

$27.00

Fill Volume

100 mL

COA
COA

21221

3M Potassium Acetate, pH 5.5, 100mL, Sterile

Quantity

Minimum is set to 10 for this product*

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Description
Formulation
Common Applications
Storage

3M Potassium Acetate, pH 5.5 is a ready-to-use aqueous salt solution for pH control and salt-dependent precipitation in molecular biology and biochemistry. The acetate buffer at pH 5.5 provides conditions suitable for neutralization of alkaline cell lysates and selective precipitation of proteins and detergent complexes during plasmid DNA isolation from E. coli.

Applications include RNA precipitation, DNA cleanup, and use as a buffering component in enzymatic reactions requiring acetate at pH 5.5. Compatible with downstream procedures such as electrophoresis, cloning, and qPCR following appropriate purification.

Supplied as a 3 M solution at pH 5.5. Ready to use; dilute only as required by specific protocols. Store at ambient temperature and protect from contamination. For laboratory use.

  • 3M Potassium Acetate

  • Plasmid Miniprep Neutralization: 3M Potassium Acetate, pH 5.5 is used in alkaline lysis to neutralize lysates and precipitate SDS, proteins, and chromosomal DNA during plasmid prep from Escherichia coli.

  • DNA/RNA Precipitation and Cleanup: Serves as the salt source to facilitate ethanol or isopropanol precipitation of nucleic acids and removal of contaminants.

  • SDS Removal: Precipitates SDS as insoluble potassium dodecyl sulfate, clarifying samples prior to electrophoresis or spectrophotometric analysis.

  • Acetate Buffering for Enzyme Assays: Provides a defined pH 5.5 environment for acid-active enzymes and nucleic acid–related assays requiring acetate buffer.

  • Nucleic Acid Extraction Workflows: Added after phenol–chloroform extraction to adjust ionic strength and pH, improving DNA recovery and purity.

Storage Temperature
Room Temperature
Shelf Life
1095 days
Plasmid Workflow

This product is part of our Plasmid Workflow

To expedite your plasmid production, we offer a wide variety of high-quality off-the-shelf and custom solutions, from selective culture media to buffers and reagents that can be used throughout the pDNA growth and purification process.

  • Selection and Culturing
  • Purification and Recovery

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