21220

E. coli Lysis Solution, 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$83.00

Fill Volume

1000 mL

See label

COA

21220

E. coli Lysis Solution, 1000mL, Sterile.

Quantity

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Description

Formulation

Common Applications

Storage

E. coli Lysis Solution is an alkaline detergent reagent for cell lysis in plasmid DNA preparation workflows from E. coli cultures. It disrupts cell envelopes and promotes alkaline denaturation of chromosomal DNA and proteins, enabling separation of plasmid DNA following neutralization and clarification.

The solution is suitable for use in standard alkaline lysis procedures and is compatible with downstream purification methods, including silica-based and anion-exchange-based plasmid isolation protocols. Intended for laboratory use with E. coli cultures grown in common rich media.

0.2M Sodium Hydroxide
1.0% (w/v) SDS

Plasmid DNA Miniprep (Alkaline Lysis): Rapid lysis of E. coli cells for isolation of plasmid DNA using SDS/NaOH alkaline lysis workflows.
Midi/Maxi/BAC Plasmid Preparation: Scalable alkaline lysis of E. coli cultures for high-yield plasmid, cosmid, or BAC purification.
Colony PCR Template Prep: Generation of crude lysates from E. coli colonies to provide DNA templates for PCR screening.
Sanger Sequencing Template Prep: Quick lysis of E. coli colonies to obtain plasmid-rich lysates for direct sequencing workflows.
Cloning and Screening Workflows: Lysis step prior to neutralization/clearing for restriction analysis, ligation verification, and construct validation from E. coli cultures.
qPCR/ddPCR Screening: Preparation of crude DNA lysates from E. coli for quantitative PCR-based clone verification.
High-Throughput Automation: Plate-based alkaline lysis of E. coli in 96-/384-well formats for parallel plasmid screening.

Storage Temperature

Room Temperature

Shelf Life

1095 days

Plasmid Workflow

This product is part of our Plasmid Workflow

To expedite your plasmid production, we offer a wide variety of high-quality off-the-shelf and custom solutions, from selective culture media to buffers and reagents that can be used throughout the pDNA growth and purification process.

Selection and Culturing
Purification and Recovery

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E. coli Lysis Solution, 1000mL, Sterile.

Certificate of Analysis

This product is part of our Plasmid Workflow

Looking for a Certificate of Analysis?

A trusted partner that

can help you scale

Raising the bar on quality

Delivery in weeks, not months

Add our expertise to your team

We're custom by default

Making solutions possible