Plasmid DNA Extraction (Alkaline Lysis): Rapid alkaline SDS lysis of E. coli cultures for miniprep/midiprep plasmid purification.

High-Throughput Plasmid Screening: 96-well/automation-friendly lysis of E. coli for parallel plasmid prep workflows.

Colony PCR Preparation: Quick lysis of E. coli colonies to generate DNA templates for PCR screening.

Crude Lysate for Sanger/NGS: Preparation of plasmid-containing lysates from E. coli for sequencing workflows.

Bacterial Strain Verification: Alkaline SDS lysis of E. coli to obtain DNA for confirming inserts or edits by PCR.

Transfection-Grade Plasmid Prep (Upstream Step): Initial alkaline lysis of E. coli prior to downstream plasmid cleanup/polishing.

Library Cloning Workflows: Lysis of recombinant E. coli for rapid screening and recovery of cloned plasmid constructs.

EndA+ Strain Handling: Robust NaOH/SDS lysis of E. coli strains with nucleases to facilitate plasmid recovery.