Plasmid DNA Resuspension: Rehydrates and solubilizes purified plasmid DNA pellets for downstream use.

Plasmid Storage: Provides pH 8.0 Tris and EDTA chelation (50 mM Tris/Tris-HCl, 10 mM EDTA) to protect DNA from degradation during short- and long-term storage.

PCR/qPCR Template Preparation: Serves as a stable diluent for plasmid templates to ensure consistent amplification.

Sanger/NGS Sequencing: Maintains DNA integrity and compatibility for accurate sequencing reads.

Restriction Enzyme Digestion: Suitable for diluting DNA prior to digestion; buffering capacity supports reliable setup when reaction buffer supplies Mg2+.

Cloning and Ligation Workflows: Used to prepare plasmid inserts or vectors for ligation, Gibson/HiFi assembly, and downstream transformations into Escherichia coli.

Normalization and Aliquoting: Enables consistent concentration adjustments and aliquot preparation for reproducible experiments.

DNA Quantification Standards: Provides a defined matrix for spectrophotometric or fluorometric calibration and sample dilution.

Transfection Prep: Common resuspension medium for plasmids destined for mammalian cell transfection workflows.

Archiving and Shipping: Protects plasmid DNA stability during sample transport and repository storage, including strains carrying plasmids in E. coli.