Plasmid DNA Resuspension: Rehydrates and solubilizes purified or lyophilized plasmid DNA pellets for downstream use.

DNA Storage: Maintains pH 8.0 and chelates divalent cations (EDTA) to protect plasmid DNA from nuclease degradation during short- and long-term storage.

Restriction Enzyme Digests: Provides a stable buffer environment for reliable endonuclease digestion of plasmid DNA.

Ligation and Cloning Workflows: Preserves plasmid integrity prior to ligation, cloning, and preparation for transformation into E. coli.

PCR/qPCR Templates: Serves as a compatible diluent for plasmid DNA templates in amplification assays.

Sanger/NGS Sequencing Prep: Buffers plasmid DNA for sequencing submissions and library construction.

DNA Quantification and Normalization: Used as a reference/blank and diluent for spectrophotometric and fluorometric measurements.

Oligo/Plasmid Reconstitution: Dissolves lyophilized plasmids and relevant DNA constructs for experimental setup.