Plasmid Miniprep Resuspension (Buffer P1 equivalent): Used to resuspend E. coli cell pellets prior to alkaline lysis during pDNA isolation (50 mM Tris/Tris-HCl, 10 mM EDTA supports pH control and nuclease inhibition).

Midiprep/Maxiprep Cell Pellet Resuspension: Applied to larger-scale E. coli pellets to ensure uniform lysis and high-quality plasmid yields.

pDNA Pellet Reconstitution: Dissolves ethanol-precipitated or lyophilized plasmid DNA for handling and storage while protecting from nuclease activity.

Short-Term pDNA Storage: Maintains plasmid integrity at pH 8.0; EDTA chelation minimizes metal-dependent nuclease degradation.

Standardization of Plasmid Stocks: Provides a consistent buffer matrix for preparing reference plasmid solutions for cloning and analytical workflows.

BAC/Cosmid Preparation: Resuspends large plasmid-bearing E. coli pellets prior to gentle lysis to preserve high–molecular-weight DNA.

Sample Transport and Archiving: Serves as a protective carrier for plasmid DNA during short-term shipment or bench-top storage.

Kit Reagent Replacement: Functions as a ready-to-use substitute for resuspension buffers in plasmid prep kits requiring 50 mM Tris/Tris-HCl, 10 mM EDTA.