20mM Histidine with 8% Sucrose, pH 5.5 is a ready-to-use formulation buffer of histidine and sucrose in WFI quality water. Histidine buffers the solution at pH 5.5, and 8% sucrose acts as a non-ionic stabilizer that brings the buffer close to isotonic. The 1 L fill supports laboratory and pilot-scale formulation work.

It is commonly used as a diafiltration, holding, and formulation buffer for proteins and biologics, including monoclonal antibodies and ADCs. Exchange purified protein into the buffer by TFF or dialysis, then add surfactant as needed. Sucrose protects the protein through freezing and freeze-drying, which makes the buffer a practical baseline for lyophilization cycle development.