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20777

AAV Affinity Load High Conductivity Adjustment Solution

Availability

Ships in 6 - 14 Days

Container Size

125mL

Item Count

1 Bottle(s)

Price per Item

$66.00

Fill Volume

100 mL

20777

AAV Affinity Load High Conductivity Adjustment Solution

Quantity

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Description
Formulation
Common Applications
Storage

AAV Affinity Load High Conductivity Adjustment Solution is a high-ionic-strength chloride salt solution intended to increase sample conductivity prior to loading adeno-associated virus preparations onto affinity chromatography resins. It is used to adjust ionic strength without altering buffer composition, enabling preparation of feedstreams to target conductivity ranges recommended for AAV capture steps. The solution is compatible with common buffering systems used in AAV workflows and with a broad range of serotypes.

This product is ready-to-use, sterile-filtered, nuclease-free, protein-free, and does not contain buffering agents. Typical use involves incremental addition to clarified AAV samples or equilibration buffers to reach the desired conductivity, followed by verification with a calibrated conductivity meter. Store at 2–8 °C. Do not freeze.

  • 5M Sodium Chloride
  • AAV Affinity Load Conditioning: Raises sample ionic strength with 5 M Sodium Chloride to reach target conductivity before loading onto AAV affinity resins.

  • Nonspecific Binding Suppression: Uses high salt (5 M Sodium Chloride) to minimize host cell protein and nucleic acid interactions during capture.

  • Capture Selectivity and Yield Optimization: Tunes conductivity with 5 M Sodium Chloride to favor AAV binding while reducing co-capture of impurities.

  • Column Protection and Fouling Reduction: Maintains higher ionic strength with 5 M Sodium Chloride to limit resin fouling and carryover.

  • Method Development and Titration: Enables stepwise or on-the-fly conductivity adjustments using 5 M Sodium Chloride during DoE and process optimization.

Storage Temperature
Room Temperature
Shelf Life
1095 days
AAV Chromatography Workflow

This product is part of our AAV Chromatography Workflow

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