AAV Affinity Chromatography Loading: Conditions clarified harvest to low conductivity and appropriate pH with 20 mM Tris base to promote AAV capsid binding to affinity resins.

Sample Dilution/Conditioning: Dilutes high-salt feeds to target ionic strength before the capture step, minimizing breakthrough during loading.

Buffer Exchange/Desalting Aid: Facilitates salt reduction and transition into a low-conductivity matrix prior to AAV affinity capture.

pH Stabilization: Uses 20 mM Tris base to maintain consistent pH during load preparation, supporting AAV stability and binding.

Process Development: Employed in scouting conductivity/pH conditions and determining dynamic binding capacity for various AAV serotypes.

Nuclease Treatment Compatibility: Maintains suitable pH/ionic strength during endonuclease treatment steps prior to affinity capture.

Feedstream Standardization: Harmonizes variability between batches or upstream feeds by normalizing conductivity before column loading.

Scale-Up Support: Used in pilot and GMP operations to reproducibly condition large-volume AAV loads for affinity purification.