AAV Affinity Elution Buffer is an acidic buffer formulated for the elution step in affinity chromatography workflows for adeno-associated virus (AAV) capsids. It is intended to release AAV particles from affinity media that require low-pH conditions for desorption, supporting purification processes across serotypes that elute under acidic conditions.

During use, the buffer is applied to the affinity column after loading and washing to recover bound AAV. Eluate fractions are typically collected into, or promptly adjusted with, a suitable neutralizing buffer to return the sample to near-physiological pH. The buffer is compatible with downstream processing steps, including buffer exchange, desalting, tangential flow filtration, and ion-exchange chromatography.