Affinity Column Equilibration (AAV): Equilibrates AAV affinity resins/columns before sample loading to establish 20 mM Tris, 0.4 M NaCl, pH 7.5 conditions.

Sample Conditioning/Buffer Exchange: Dilutes or exchanges clarified AAV harvest into binding conditions to maximize capture on affinity media.

Column Wash: Used as the primary wash buffer to remove host-cell proteins, DNA, and process impurities while maintaining capsid stability.

Diluent for Load and Standards: Adjusts ionic strength and pH of process intermediates, standards, and controls for consistent chromatography performance.

System Priming and Baseline Stabilization: Primes chromatography/skid flow paths and stabilizes UV baseline prior to AAV affinity capture runs.

Serotype-Agnostic Capture Workflows: Provides a neutral, high-salt environment compatible with common AAV serotypes during capture.