AAV Affinity Column Cleaning-in-Place (CIP): High-strength 6 M guanidine hydrochloride removes tightly bound impurities and fouling from AAV affinity resins between cycles.

Resin Regeneration: Restores capacity by denaturing and clearing host cell proteins, nucleic acids, and lipids from the resin matrix.

Aggregate and Precipitate Removal: Solubilizes AAV aggregates and protein precipitates on resin, frits, and hardware surfaces.

Endotoxin and Bioburden Reduction: Reduces endotoxin load and microbial bioburden (e.g., Escherichia coli, Pseudomonas aeruginosa); not a sterilant.

Sanitization of Downstream Hardware: Flush for compatible pumps, tubing, and valves to remove proteinaceous soils and carryover.

Carryover Mitigation: Minimizes cross-contamination between purification campaigns and product lots.

Cleaning Validation Studies: Used as a worst-case chaotropic agent to challenge and verify protein removal in CIP protocols.

Nucleic Acid Decontamination: Disrupts DNA/RNA interactions to clear residual host DNA and plasmid contaminants from columns.