AAV Affinity Column Cleaning-in-Place (CIP): 0.1 M phosphoric acid formulation used to strip fouling and residual product from AAV affinity resins between purification cycles.

Removal of Process Impurities: Disrupts ionic and electrostatic interactions to remove host cell proteins, residual DNA/RNA, and lipidic contaminants from affinity media.

Endotoxin Burden Reduction: Acidic wash helps reduce endotoxins originating from Gram-negative hosts such as Escherichia coli during downstream processing.

Bioburden Control/Sanitization: Low-pH exposure contributes to inactivation of adventitious microbes and bioburden control on packed affinity columns.

Column Regeneration and Performance Recovery: Restores dynamic binding capacity and reduces carryover by clearing tightly bound aggregates and foulants.

Between-Campaign Maintenance: Applied after elution and prior to re-equilibration to prepare columns for subsequent AAV purification runs.