AAV Affinity Chromatography Wash: High-salt wash during capture to remove nonspecific proteins and nucleic acids while keeping AAV bound to the resin.

Column Equilibration and Conditioning: Used to equilibrate and condition AAV affinity media prior to sample loading for consistent binding performance.

Post-Load Cleansing Wash: Applied after sample loading to clear weakly bound host cell proteins and DNA without eluting AAV.

Sample Dilution/Adjustment: Mixed with lysate or supernatant to set ionic strength and pH before affinity capture.

Resin Specificity Enhancement: Minimizes nonspecific interactions on AVB/AAVX and similar AAV affinity resins to improve purity.

Serotype-Agile Processing: Compatible wash for multiple AAV serotypes during affinity capture workflows.

Carryover Reduction Between Runs: Used between cycles to limit residual contaminants and improve run-to-run consistency.

Endotoxin and Bioburden Mitigation: Helps reduce carryover of endotoxin and microbial impurities originating from E. coli-derived materials during wash steps.