AAV Affinity Chromatography Elution: Elutes recombinant AAV from affinity resins (e.g., AVB, AAVX) using low-pH 0.1M acetic acid/0.4M sodium chloride, pH 2.75.

Impurity Clearance During Elution: Disrupts nonspecific interactions to reduce host-cell protein and DNA carryover at the affinity step.

FPLC/LC System Elution: Pre-formulated elution buffer for automated systems (e.g., ÄKTA) to achieve reproducible column elution profiles.

Serotype and Process Development: Scouting elution conditions across AAV serotypes to optimize residence time, fractionation, and neutralization strategy.

Scale-Transition Purification: Ready-to-use sterile buffer supporting consistent AAV elution from small-scale to manufacturing workflows.

Acidic Fraction Collection with Rapid Neutralization: Collection of low-pH elution fractions followed by immediate neutralization to protect vector integrity.

Downstream Buffer Exchange Compatibility: Facilitates subsequent neutralization, TFF, and buffer exchange into formulation buffers after elution.