Affinity Column Equilibration: Pre-equilibrates AAV affinity resins to 20 mM Tris, 0.4 M NaCl, pH 7.5 for consistent capsid binding.

Sample Conditioning: Adjusts clarified harvest to the specified Tris/NaCl/pH conditions prior to loading to maximize capture efficiency.

Binding/Loading Buffer: Used during sample application to promote selective interaction of AAV capsids with affinity ligands.

Column Wash: Removes host-cell proteins, DNA, and process impurities while retaining AAV on the resin under 20 mM Tris, 0.4 M NaCl, pH 7.5.

System Conditioning/Blanking: Primes FPLC/ÄKTA flow paths and establishes UV baseline with a sterile, composition-matched buffer.

In-Process Hold Buffer: Provides short-term capsid stability between purification steps at neutral pH and high ionic strength.