AAV Clarification and Sterile Filtration: Used as a process buffer to clarify and sterile-filter AAV preparations while maintaining capsid integrity.

Tangential Flow Filtration (TFF)/Diafiltration: Serves as the exchange buffer during AAV concentration and diafiltration for consistent ionic strength and pH control.

Formulation and Dilution of AAV: Provides a final formulation/dilution matrix (20 mM Tris, 0.2 M NaCl, 1.0% sucrose, 0.1% Poloxamer 188, pH 8.0) to stabilize vectors for downstream use.

Capsid Stabilization During Processing: Sucrose (1.0%) helps protect AAV capsids from shear and osmotic stress throughout filtration steps.

Reduced Adsorption and Improved Recovery: Poloxamer 188 (0.1%) minimizes AAV loss to tubing, filters, and vessel surfaces to enhance yield.

Chromatography Support: Used as an equilibration/wash buffer around pH 8.0 with moderate salt (0.2 M NaCl) during ion-exchange capture or polishing of AAV.

Sample Preparation for Analytical Assays: Maintains native conditions for qPCR/ddPCR titering, ELISA, and infectivity assays following filtration steps.

Membrane Conditioning and Wetting: Conditions virus-grade filters prior to load to reduce non-specific binding and improve throughput.

Intermediate Hold Buffer: Stabilizes AAV during short-term holds between unit operations at controlled temperature.

Process-Compatible Diluent: Used to adjust conductivity and pH of in-process pools without introducing denaturing components.