Protein Extraction from Mammalian Cells: Efficient lysis of adherent and suspension cells for total protein recovery using a 10X buffer (500 mM Tris, 2.0 M NaCl, 5.0% Tween-20, pH 8.5).

Western Blot Sample Preparation: Generates clarified lysates compatible with SDS-PAGE and immunoblotting workflows.

ELISA and Immunoassay Prep: Produces detergent-solubilized protein samples suitable for antigen quantification.

Immunoprecipitation/Co-IP: Provides high-ionic strength, non-ionic detergent conditions to preserve protein–protein interactions during pull-downs.

Nucleic Acid Preparation: Cell lysis prior to DNA/RNA purification from mammalian cultures for downstream PCR/qPCR.

Reporter Assays: Lysis for luciferase, GFP, and β-galactosidase measurements from transfected cells.

Membrane Protein Solubilization: Mild non-ionic detergent facilitates extraction of membrane-associated proteins without denaturation.

Virus and VLP Release: Liberation of enveloped viral particles and VLPs from producer cell lines for titering or purification.

Phosphoprotein/Kinase Assays: High-pH, high-salt conditions compatible with preserving phosphorylation states for signaling studies.

Large-Volume Harvests: 100 mL of 10X concentrate is sufficient to process lysates from up to 1 L of cell culture harvest.