Yeast Cultivation and Propagation: General-purpose growth of Saccharomyces cerevisiae and other yeasts in a rich, non-selective medium (2.0% Peptone, 1.0% Yeast Extract, 2.0% Glucose).

Starter Cultures for Fermentation: Rapid biomass buildup for inoculating fermenters and shake-flask experiments.

Transformation Recovery/Outgrowth: Post–lithium acetate or electroporation recovery to maximize viability before selection.

Growth Curves and Physiology Studies: Monitoring doubling times, glucose repression effects, and stress-response assays in rich media.

Protein Expression in Yeast: Non-selective pre-cultures for recombinant protein production and secretion studies.

Mating and Genetic Crosses: Non-selective outgrowth for mating efficiency tests and tetrad-related workflows.

Colony Formation and CFU Enumeration: Preparing dense, healthy cultures for plating (with YPD agar) and viability counts.

Glycerol Stock Preparation: Generating robust log-phase cultures for long-term cryopreservation.

Yeast Two-Hybrid Workflows: Pre-culturing bait/prey strains prior to selective assays on defined media.

Antifungal Research Pre-Culture: Growing yeast isolates prior to susceptibility testing on standardized media.