Yeast Cultivation and Colony Isolation: Rich, non-selective medium for robust growth and single-colony isolation of Saccharomyces and other fungi.

Strain Maintenance and Stocking: Routine streaking, passaging, and plate storage of laboratory yeast strains.

Transformation Recovery: Post-transformation outgrowth prior to selection on defined or drug-containing media.

Replica Plating and Genetic Screens: Initial growth for phenotypic, temperature-sensitive, and suppressor/synthetic-lethal screens.

Mating and Ploidy Workflows: Pre-growth and lawn formation for mating assays and subsequent ploidy analyses.

Drug/Stress Spot Assays: Comparative growth under antifungals or stressors for sensitivity profiling.

Viability and CFU Enumeration: Quantification of viable yeast by plate counts and dilution spotting.

Fermentation Phenotype Checks: Rapid assessment of growth and morphology on glucose-rich plates.

Quality Control of Yeast Cultures: Monitoring culture purity and consistency during strain construction.

Enzyme and Reporter Assays: Plate-based readouts (e.g., colorimetric/fluorescent reporters) after growth on rich medium.