High-Density Bacterial Culture: Promotes robust growth of E. coli using 0.4% glycerol as carbon source and 17/72 mM phosphate buffering.

Recombinant Protein Expression: Supports high-yield expression in E. coli T7/lac systems with stable pH and rich 2.0% animal-free soytone + 2.4% yeast extract.

Plasmid DNA Production: Enables high-copy plasmid amplification and preparation from dense E. coli cultures.

Transformation Recovery/Outgrowth: Enhances post-heat shock/electroporation recovery of E. coli prior to plating or selection.

Antibiotic Selection and Clone Screening: Used with appropriate antibiotics for selection and colony screening of engineered E. coli.

Phage Propagation/Phage Display: Suitable for M13 and related phage workflows in E. coli due to high-cell-density growth.

Starter Cultures for Bioreactors: Generates concentrated inocula for scale-up from shake flasks to fermenters.

Glycerol Stock Preparation: Produces dense E. coli cultures ideal for long-term cryostorage stocks.

Library Amplification: Supports outgrowth and amplification of plasmid and genomic libraries in E. coli.