Cell and Tissue Washing: Isotonic rinsing of cells and tissues to minimize osmotic stress during handling and processing.

Sample Dilution and Resuspension: Diluting and resuspending cell pellets and microbial colonies (e.g., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa) for downstream analyses.

Microbiological Assays: Preparing saline suspensions for colony counting, serial dilutions, and antimicrobial susceptibility testing.

Reagent Reconstitution: Reconstituting lyophilized standards, controls, and reference materials where a simple isotonic solution is required.

Spectrophotometry and Turbidity Controls: Preparing blanks and standard suspensions for OD and turbidity measurements.

Flow Cytometry Sample Medium: Maintaining isotonic conditions for cell suspensions during analysis and sorting.

General Rinsing in Aseptic Workflows: Sterile rinsing of labware, instruments, and filtration devices to remove residual reagents.

Osmotic Balance in Assays: Adjusting ionic strength in biochemical and diagnostic workflows that require 0.9% Sodium Chloride.