DNA and RNA Extraction: The 10mg/mL Proteinase K Solution digests proteins and inactivates DNases/RNases to release high-quality nucleic acids from cells, tissues, Escherichia coli, Staphylococcus aureus, yeast, and fungi.

Genomic DNA from Tough Microbes: Facilitates lysis (often with SDS/EDTA) to recover DNA from robust organisms such as Bacillus subtilis and Staphylococcus aureus.

Plasmid Preparation: Degrades protein contaminants after alkaline lysis, improving plasmid yield and purity.

RT-PCR/NGS Sample Preparation: Removes proteins and nucleases prior to reverse transcription and library construction, enhancing RNA quality.

Viral RNA Extraction: Aids proteolysis and nuclease inactivation in clinical and environmental samples for RT-qPCR assays.

FFPE and Tissue Processing: Releases nucleic acids from formalin-fixed, paraffin-embedded tissues and biopsies via proteolysis.

Forensic and Ancient DNA: Liberates DNA from challenging substrates (hair, bone, touch DNA) by digesting keratin and collagen.

Microbiome and Environmental Samples: Breaks down protein matrices to free nucleic acids from complex samples (soil, feces, wastewater), including targets like Escherichia coli.

Nuclease Inactivation in Lysates: Protects nucleic acids by deactivating RNases and DNases during extraction workflows.

Inhibitor Reduction for PCR: Removes proteinaceous inhibitors prior to amplification to improve PCR success.