Routine Cultivation of Escherichia coli: General growth of recombinant and lab strains in LB Miller formulation (1.7% Soytone, Animal-Free; 0.5% Yeast Extract; 1.0% NaCl).

Plasmid Cloning and Propagation: Overnight cultures for high-yield plasmid prep and antibiotic selection in E. coli.

Transformation Recovery/Outgrowth: Post heat-shock or electroporation recovery of E. coli prior to plating or selection.

Starter Cultures for Protein Expression: Seed and shake-flask cultures of E. coli prior to induction in richer media.

Glycerol Stock Preparation: Production of stable freezer stocks from healthy E. coli overnight cultures.

Preparation of LB Agar Plates: Base broth for plate media by adding agar for selection and colony screening.

Blue/White Screening Workflows: Broth for overnight culture from screened colonies carrying lacZ-based reporters.

Bacteriophage Work: Growth of E. coli host strains for phage propagation and titering.

Antibiotic Susceptibility/Selection Assays: Maintenance and selection of plasmid-bearing E. coli with appropriate antibiotics.

Animal-Free Process Development: Media for workflows requiring animal-free components in QC and regulated environments.