Studier Induction ZYM-5052 Medium

Studier Induction medium is based on Studier’s ZYM-5052 medium and has been reformulated for ease of use. All of the ingredients are contained in 2 bottles instead of the usual 5.

At the time of usage, aseptically add 60 mL of Studier Salts Solution (#3S2600, included) to Studier ZYM-5052 Medium for Induction (#3S2200, included).
Media is stable for 2 months once the salt solution has been added. This media contains glucose, lactose, and glycerol as carbon sources. No further additions are required. Induction media works with all T7 regulated E. coli vectors in BL21 and derivative strains.

Reference: F.W. Studier (2005) Protein production by auto-induction in high-density shaking cultures. Prot. Exp. Pur. 41, 207-234.