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Y5410

YPG Broth, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$112.00

Fill Volume

1000 mL

Y5410

YPG Broth, Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

YPG Broth is a nutrient broth formulated with tryptone, yeast extract, and D-(+)-galactose for cultivation and maintenance of yeast under galactose conditions.

The galactose serves as a fermentable carbon source and is suitable for workflows requiring galactose-dependent gene expression.

  • 2.00% Tryptone
  • 1.00% Yeast Extract
  • 2.00% D-(+)-Galactose
  • Yeast Cultivation and Maintenance: Supports robust growth of galactose-utilizing yeasts (e.g., Saccharomyces cerevisiae) with 2.00% Tryptone, 1.00% Yeast Extract, and 2.00% D-(+)-Galactose.

  • GAL Promoter Induction: Enables induction of GAL-regulated gene expression systems for recombinant protein production and functional studies.

  • Galactose Metabolism Studies: Used to assay GAL gene function, screen galactose utilization mutants, and analyze carbon source switching.

  • Yeast Transformation Recovery: Provides a rich, non-glucose medium for post-transformation recovery and expression of GAL-driven selection markers.

  • Metabolic Flux and Transcriptomic Assays: Facilitates profiling under galactose conditions to compare fermentative versus alternative carbon source responses.

  • Strain Propagation for Functional Assays: Prepares starter cultures for downstream phenotyping, enzymatic assays, and growth kinetics on galactose.

  • Fermentation Process Development: Evaluates production performance and pathway engineering outcomes using galactose as the primary carbon source.

  • Quality Control of Yeast Stocks: Routine passaging and viability checks in a defined rich medium free of glucose repression effects.

  • pH: 6.50 ± 0.10
Storage Temperature
Room Temperature
Shelf Life
730 days
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