Yeast Culture and Colony Isolation: Nonselective growth and single-colony purification of Saccharomyces cerevisiae and other yeasts on rich medium (1.0% Yeast Extract, 2.0% Peptone, 2.0% Glucose, 2.5% Agar).

CFU Enumeration and Viability Testing: Quantification of viable yeast cells via colony counts.

Transformation Recovery and Outgrowth: Post-transformation recovery prior to selection or replica plating.

Growth Phenotyping and Spot Assays: Comparative fitness, temperature sensitivity, and drug-response spot tests.

Antibiotic/Drug Selection Base: Supplementation with G418, nourseothricin, or hygromycin B for selectable marker screening in yeast.

Mating and Patching: Mating assays, diploid selection steps, and routine patching or streaking for strain maintenance.

Replica Plating and Library Handling: Transferring colonies to selective media and managing mutant or deletion libraries.

Fermentation Strain Screening: Rapid plate-based evaluation of production strains and adaptive evolution lineages.

Contamination Assessment: Visual detection of microbial contaminants in yeast cultures, including potential growth of Escherichia coli if present.

Teaching and Training: General microbiology and yeast genetics exercises and plate technique practice.