Yeast Cultivation and Maintenance: Routine growth and propagation of Saccharomyces and other non-pathogenic yeasts on a rich, nonselective medium.

Colony Isolation and Enumeration: Streaking, single-colony isolation, and CFU counting for yeast cultures.

Post-Transformation Recovery: Recovery of yeast after chemical, electroporation, or CRISPR editing prior to selection.

Phenotypic Screening: Assessing growth differences, temperature sensitivity, and drug responses (with supplements as needed).

Replica Plating Master Plates: Preparing dense master plates for subsequent replica transfer onto selective media.

Fermentation Strain Screening: Preliminary screening and comparison of industrial or laboratory yeast strains for performance traits.

Yeast Viability and Fitness Assays: Monitoring viability, growth rates, and competitive fitness in rich conditions.

Germination and Recovery of Spores: Plating dissected tetrads or spores for germination and colony outgrowth.

Short-Term Culture Storage: Maintaining patches or lawns for short-term storage and routine passaging.

General Fungal Cultivation: Nonselective culture of various yeasts and fungi requiring a nutrient-rich medium.