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X1205

X-Gal. 5g

Availability

Ships in 6 - 14 Days

Container Size

60cc

Item Count

1 Dry Container(s)

Price per Item

$371.00

Fill Volume

5 g

COA
COA

X1205

X-Gal. 5g

Quantity

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Description
Formulation
Common Applications
Storage

X-Gal is a chromogenic substrate for β-galactosidase used to visualize lacZ activity. In cloning workflows, it enables blue-white screening of recombinant colonies via α-complementation of lacZ on agar plates supplemented with IPTG and a selective antibiotic, allowing visual differentiation of insert-containing transformants in E. coli.

Typical working concentrations range from 20–40 µg/mL in agar media. Prepare solutions in an appropriate solvent, protect from light, and store at low temperature to maintain stability. Applications include colony screening, reporter assays, and detection of β-galactosidase activity in E. coli and related systems.

  • X-Gal
  • Ultra pure grade

  • Blue/White Colony Screening: Chromogenic substrate for lacZ α-complementation on LB agar with IPTG to distinguish recombinants in E. coli.

  • β-Galactosidase Reporter Staining: Histochemical detection of lacZ expression in cells and tissues (bacterial, yeast, and mammalian systems).

  • Phage Plaque Screening: Visualization of recombinant plaques in lacZ-based bacteriophage libraries (e.g., M13/λ) using X-Gal/IPTG.

  • Bacmid Selection: Blue/white screening of recombinant bacmids in E. coli DH10Bac for Bac-to-Bac baculovirus workflows.

  • Colony/Filter Lift Assays: On-plate overlays or membrane lifts to assay β-galactosidase activity from E. coli and other hosts.

  • Bacterial/Yeast Two-Hybrid Readouts: lacZ-based reporter assays where X-Gal provides chromogenic detection of interaction-dependent β-gal activity.

Storage Temperature
Frozen
Shelf Life
days
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