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T5594

50mM Tris pH 7.6, 1mM EDTA pH 8.0, 0.5% Tween-20. 10mL Tubes, Sterile, RNAse Tested. 25 Pack

Availability

Ships in 6 - 14 Days

Container Size

13mL

Item Count

25 Tube(s)

Price per Item

$146.00

Fill Volume

10 mL

T5594

50mM Tris pH 7.6, 1mM EDTA pH 8.0, 0.5% Tween-20. 10mL Tubes, Sterile, RNAse Tested. 25 Pack

Quantity

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Description
Formulation
Common Applications
Storage

This is a buffered, non-ionic detergent solution used for washing steps, sample preparation, and general molecular biology workflows. Tris maintains near-neutral pH, EDTA chelates divalent cations to reduce nuclease activity, and Tween-20 provides mild surfactant properties for efficient rinsing of membranes, beads, and cells.

RNase Tested. Suitable for use in nucleic acid extraction and cleanup workflows, Western and dot blot washing steps, and routine handling of bacterial samples such as E. coli.

  • 50mM Tris (pH 7.6)
  • 1mM EDTA (pH 8.0)
  • 0.5% Tween-20
  • Nucleic Acid Resuspension and Storage: Protects DNA/RNA from nuclease activity via EDTA while Tween-20 minimizes adsorption to plastic surfaces.

  • qPCR/RT-qPCR Template Dilution: Provides stable pH and reduces surface binding/inhibitors for consistent amplification performance.

  • RNA Handling Workflows: RNase-tested buffer for RNA sample dilution, normalization, and short-term storage.

  • Nucleic Acid Extraction Washes: Used as a low-ionic-strength wash to reduce nonspecific binding during bead-based or column cleanups.

  • ELISA and Immunoassay Plate Washes: Tris-based Tween-20 buffer to minimize nonspecific interactions during washing steps.

  • Western Blot Washes: Detergent-containing Tris buffer for membrane washing to reduce background binding.

  • Library Prep and Bead-Based Workflows: Dilution/wash buffer for DNA cleanup steps to enhance recovery and consistency.

  • Enzyme Reaction Diluent: Maintains near-neutral pH for nucleic acid–related enzymes while limiting nuclease activity via metal chelation.

  • Flow Cytometry/IF Washes: Mild detergent buffer for gentle cell/permeabilization washes to reduce nonspecific staining.

  • Sample Transport/Dilution for Swabs: Low-ionic-strength, detergent-containing buffer used to elute and stabilize nucleic acids prior to analysis.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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