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T3456

10X TE Buffer Solution. DNase/RNase Tested, PCR Grade. 100mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

125mL

Item Count

1 Bottle(s)

Price per Item

$53.00

Fill Volume

100 mL

T3456

10X TE Buffer Solution. DNase/RNase Tested, PCR Grade. 100mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

10X TE Buffer Solution, DNase/RNase Tested, PCR Grade is a concentrated Tris–EDTA buffer used for dilution, resuspension, and storage of DNA and RNA in molecular biology workflows. The chelating component helps limit divalent cation availability during nucleic acid handling.

Each lot is tested for DNase and RNase activity, and PCR-grade preparation is intended to reduce introduction of amplification inhibitors. Supplied at 10X concentration for preparation of working solutions by dilution with nuclease-free water.

  • 100mM Tris
  • 10mM Ethylenediaminetetraacetic acid (EDTA)
  • Nucleic Acid Storage: Long-term storage of plasmid DNA, genomic DNA, and RNA; 100 mM Tris and 10 mM EDTA maintain pH and chelate divalent cations to inhibit nucleases.

  • DNA/RNA Resuspension and Dilution: Rehydration of lyophilized nucleic acids and dilution of templates for PCR/qPCR while preserving integrity.

  • PCR/qPCR Sample Preparation: Stable pH buffer for template handling and dilution in PCR-grade, DNase/RNase-tested workflows.

  • DNA Elution Buffer: Elution of DNA from silica spin columns and gel extraction procedures to maximize recovery.

  • NGS Library Prep: Resuspension and storage of adapters, amplicons, and libraries to protect from nuclease degradation.

  • Cloning and Transformation: Resuspension and storage of plasmid DNA for transformation into Escherichia coli.

  • Sanger Sequencing: Preparation and stabilization of DNA templates and primers prior to sequencing.

  • Probe and Oligo Handling: Storage of oligonucleotides and labeled probes for hybridization and blotting applications.

  • pH: 8.00 ± 0.04
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1095 days
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