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T2210

DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

10L

Item Count

1 Bottle(s)

Price per Item

$366.00

Fill Volume

10000 mL

T2210

DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile.

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

DNA Suspension Buffer, pH 8.0, DNase/RNase Tested, PCR Grade is an aqueous buffer for dissolving, diluting, and storing purified DNA. The pH 8.0 buffering system supports nucleic acid stability during routine handling and storage.

DNase/RNase testing verifies the absence of detectable nuclease activity. PCR Grade specification supports downstream enzymatic workflows such as PCR, cloning, and sequencing, and is compatible with genomic DNA, plasmid DNA, and PCR amplicons.

  • 10mM Tris-HCl
  • 0.1mM Ethylenediaminetetraacetic acid (EDTA)
  • DNA Resuspension and Storage: Used to resuspend and store plasmid, genomic, or PCR amplicon DNA (e.g., plasmids from Escherichia coli), with 10 mM Tris-HCl, 0.1 mM EDTA at pH 8.0 protecting integrity.

  • PCR/qPCR Template Dilution: PCR-grade, nuclease-free buffer for diluting DNA templates while maintaining stable pH and minimizing nuclease activity.

  • NGS Library Preparation and Dilution: Used to normalize and dilute DNA libraries and adapters prior to sequencing to ensure consistency and stability.

  • Spin-Column DNA Elution: Serves as a gentle elution buffer for purified DNA to improve stability versus water-only elution.

  • Bead Cleanup Elution: Employed as an elution buffer after magnetic bead cleanups to recover DNA with improved downstream performance.

  • Cloning and Transformation Workflows: Used for resuspending ligation products and diluting DNA prior to transformation into Escherichia coli.

  • Restriction Digest Setup and Cleanup: Provides a low-salt, nuclease-tested medium for diluting DNA before or after restriction enzyme reactions.

  • Sanger Sequencing Template Prep: Used to dilute and stabilize DNA templates for reliable sequencing reads.

  • DNA Standards and Controls: Maintains consistency when preparing reference standards and controls for assay calibration.

  • Buffer Exchange/Desalting: Used to exchange DNA from incompatible buffers or salts into a stable, low-EDTA matrix suitable for diverse assays.

  • pH: 8.00 ± 0.10
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
730 days
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