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T1575

Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$100.00

Fill Volume

1000 mL

T1575

Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

Elution Solution is a low-ionic-strength Tris-HCl buffer at pH 8.5 used to elute DNA and RNA from silica-based spin columns, magnetic beads, and other solid-phase matrices.

DNase-tested to verify the absence of detectable DNase activity. Suitable as an elution, resuspension, and storage buffer for purified nucleic acids and for preparation of samples for downstream enzymatic applications.

  • 10mM Tris-HCl
  • 0.01% Tween-20
  • Silica Column DNA Elution: Elutes DNA from spin columns after PCR cleanup or gel extraction using 10 mM Tris-HCl pH 8.5 with 0.01% Tween-20 to maximize recovery and reduce adsorption.

  • Magnetic Bead Nucleic Acid Elution: Recovers DNA/RNA from SPRI or magnetic beads in library prep and cleanup workflows with a gentle 10 mM Tris-HCl pH 8.5 buffer.

  • Plasmid DNA Elution: Elutes plasmid DNA from miniprep columns prepared from Escherichia coli and other bacteria using 10 mM Tris-HCl pH 8.5 for downstream cloning or sequencing.

  • Template Preparation for qPCR/NGS: Provides a low-salt, DNase-tested, sterile 10 mM Tris-HCl pH 8.5 eluate that minimizes adsorption (0.01% Tween-20) and preserves template integrity.

  • DNA Storage and Dilution: Serves as a short- to mid-term storage/dilution buffer (10 mM Tris-HCl pH 8.5) to maintain DNA stability and reduce loss to plastics.

  • Oligonucleotide Resuspension: Reconstitutes and dilutes primers/oligos in 10 mM Tris-HCl pH 8.5 with 0.01% Tween-20 to prevent sticking to tubes and tips.

  • ChIP/ATAC-seq DNA Elution: Elutes low-input DNA from cleanup columns or beads with 10 mM Tris-HCl pH 8.5 for sensitive epigenomics workflows.

  • cDNA and Amplicon Cleanup Elution: Recovers cDNA or PCR amplicons from beads/columns into 10 mM Tris-HCl pH 8.5 to support accurate quantification and library construction.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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