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T1409

PTM1 Trace Salts Solution, 43.5mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

60mL

Item Count

1 Bottle(s)

Price per Item

$25.00

Fill Volume

43.5 mL

T1409

PTM1 Trace Salts Solution, 43.5mL, Sterile.

Quantity

Minimum is set to 25 for this product*

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Description
Formulation
Common Applications
Storage

PTM1 Trace Salts Solution is a concentrated trace element supplement for the preparation of defined media used with methylotrophic yeasts, including Komagataella phaffii (formerly Pichia pastoris). It provides trace metals needed for growth and heterologous protein expression in shake flasks and fermenters, and is commonly used with Basal Salts Medium (BSM), Buffered Glycerol-complex Medium (BMGY), and Buffered Methanol-complex Medium (BMMY).

The solution is sterile-filtered and ready to use. Add to media or feeds to achieve typical final concentrations (for example, 1 mL per liter in basal media, with adjustments as required for feed strategies). It is compatible with glycerol, glucose, and methanol carbon sources in batch, fed-batch, and continuous processes. Store refrigerated and protect from light.

  • PTM1 Trace Salts Solution
  • Fed-Batch Fermentation (Escherichia coli): PTM1 Trace Salts Solution supplies essential trace metals to support high-cell-density growth and recombinant protein production.

  • Pichia pastoris Fermentation: Used as a trace metal supplement in basal salts and induction media to enhance growth and expression performance.

  • Auto-Induction Media Preparation (Escherichia coli): PTM1 Trace Salts Solution is added to defined auto-inducing media to ensure consistent micronutrient availability.

  • Defined/Minimal Media Formulation: Provides standardized trace elements for reproducible microbial cultivation and metabolism.

  • Bioreactor Scale-Up and Process Development: Ensures consistent trace metal profiles from shake flasks to production-scale fermentors.

  • Continuous Culture (Chemostat): Maintains steady-state micronutrient levels for long-term, controlled growth studies.

  • Seed Train Development: Supplement for shake flasks and seed reactors to promote robust inoculum quality.

  • Nutrient Feed Solutions: Combined with carbon and nitrogen feeds to prevent trace metal limitations during high-demand phases.

  • High-Throughput Strain Screening: Standardizes trace metal availability across plates and microreactors for comparative studies.

  • Fermentation of Other Bacteria (Bacillus subtilis, Corynebacterium glutamicum): Supports growth and productivity by supplying essential trace elements.

Storage Temperature
Room Temperature
Shelf Life
365 days
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