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T1100

Triton X-100 Reagent Grade, 100g, Non-Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100g

Item Count

1 Bottle(s)

Price per Item

$76.00

Fill Volume

101 g

COA
COA

T1100

Triton X-100 Reagent Grade, 100g, Non-Sterile.

Quantity

Minimum is set to 10 for this product*

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Description
Formulation
Common Applications
Storage

Triton X-100, Reagent Grade is a nonionic surfactant used in laboratory workflows for cell lysis, membrane permeabilization, and protein solubilization. It is miscible with water and commonly incorporated into buffers and solutions for biochemical and cell biology procedures.

Typical applications include preparation of lysates from mammalian cells and bacteria such as E. coli, extraction of membrane-associated proteins, inclusion in wash buffers for immunoassays, and sample preparation for electrophoresis and nucleic acid workflows. Suitable for routine research use where a nonionic surfactant is required.

  • Triton X-100
  • Reagent Grade
  • Note: This product is not available for shipment to customers in the European Union.

  • Cell Lysis and Permeabilization: Gentle disruption of mammalian cells, yeast, and Escherichia coli to release cellular contents without denaturing proteins.

  • Membrane Protein Solubilization: Extraction of membrane proteins and complexes while preserving activity and native interactions.

  • Immunostaining/Immunofluorescence: Permeabilizes fixed cells and tissues to enhance antibody access for ICC/IF and IHC.

  • Western Blot/ELISA Wash Buffers: Reduces nonspecific binding and background during antibody incubation and washing steps.

  • DNA/RNA Extraction: Aids in nucleic acid purification by disrupting membranes and removing protein/lipid contaminants.

  • Virus and Envelope Disruption: Solubilizes lipid envelopes to inactivate enveloped viruses and facilitate downstream analysis.

  • Lipid Raft/DRM Studies: Used at low temperatures to isolate detergent-resistant membranes for raft analyses.

  • Additive to Buffers and Reagents: Minimizes protein aggregation and surface adsorption to plasticware during assays and storage.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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